Abstract

Grocery stores and supermarkets all sell cleaning agents. Be it used for washing of hands, body, or even cleaning the floor, all these cleaning agents ultimately have one objective -- to remove bacteria from a particular surface. Choosing the best antibacterial agent was what we wanted to find out. The choice of a good antibacterial cleaning agent would impact greatly on one’s health and living conditions. Such cleaning agents can affect the health level of the environment people work in, in terms of the cleanliness and hygiene. However, we were unable to test out all the brands in the market, and thus we chose the more reputable brands in the market. Many brands in the market claim to have superior cleaning qualities that can remove up to 99.9% of bacteria, and on many occasions, these agents do not remove up to anywhere near 99.9% of bacteria. Thus, we wanted to apply these agents in real-life testing. The question we ultimately want to answer would be “Which antibacterial agent is the best in killing bacteria?” The reason this experiment was conducted was because we wanted to prove the abilities of cleaning agents. Since many cleaning agents on the market claimed to have superior cleaning qualities that could clean and remove almost all bacteria on surfaces, we wanted to know if that was true. After some background research on the history and reputations of the cleaning agents, we chose the cleaning agents we wanted to test, which were Clorox, Dettol, Mama Lemon, and Life Buoy, as they all claimed to have superior cleaning qualities. Our hypothesis was that if Dettol was used, it could kill more bacteria than the other agents. For our experiment, we used E.coli and grew the bacteria using LB agar filled petri dishes, after serial dilution. Our chosen dilution factor was 1/100000. To test the effectivity of each cleaning agent, we grew a batch of 5 dishes of bacteria, each dish containing 100µL of bacteria sample that was spread evenly, and 10µL of each cleaning agent, which was left in the incubator overnight at 37 degree Celsius. Using the same method, we made two more batches of bacteria dishes, and similarly incubated them. After that we counted the amount of colonies in the bacteria dishes, and compared them to the number of colonies in the control, and expressed them as a percentage for easier comparison. On average, Dettol killed 33.6% of bacterial colonies.  Mama Lemon killed 35.7% of bacterial colonies. Lifebuoy killed 54.4% of bacterial colonies. Last but not the least, Clorox killed 79.1% of bacterial colonies. After analysing the results, we realised that Dettol had the most amount of bacteria colonies, which meant that few colonies were killed, and Clorox had the least number of bacteria colonies left.  We came to a conclusion that Clorox is the most effective anti-bacterial cleaning agent out of the brands (Lifebuoy, Mama Lemon & Dettol) as it can kill more bacteria than the other agents.

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